Purification of alpha-galactosidase from seeds of Sesbania marginata
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چکیده
منابع مشابه
Purification of cerebroside galactosidase from rat brain.
A method is described for the preparation of a B-galactosidase which acts on the brain lipid, ceramide galactoside. The enzyme is obtained from particles that sediment between I 000-13 ooo xg from rat brains and is extracted with a 60,& sodium cholate solution. The enzyme is solubilized and inactive proteins removed by digestion with pancreatic enzymes and the cholate is removed by dialysis and...
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The enzyme pgalactosidase from a mutant strain of A. niger UV-5 was partially purified using ammonium sulfate and acetone. The saturation range of 60-80% ammonium sulfate was found to yield 60.5% enzyme recovery with 2.4 fold purification. Acetone precipitation at enzyme: acetone ratio of 1 : 1.5 brought about a higher yield i.e. 68% and three-fold purification. The combined procedures of ...
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Two cu-galactosidase (a-o-galactoside galactohydrolase, EC 3.2.1.22) isozymes have been highly purified from human placental tissue. Both isozymes appeared to be homogeneous based upon disc gel electrophoresis. Both were bound by concanavalin A-Sepharose and stained positively with the periodic acid-Schiff stain indicating that each contained carbohydrate. The A isozyme had a molecular weight o...
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A β-galactosidase from Cicer arietinum seeds has been purified to apparent electrophoretic homogeneity using a combination of various fractionation and chromatographic techniques, giving a final specific activity of 220 units mg(-1), with approximately 1840 fold purification. Analysis of the protein by SDS-PAGE revealed two subunits with molecular masses of 48 and 38 kDa, respectively. These ba...
متن کاملFertility Control of Female Through Sesbania Sesban Seeds
Sesbania sesban (L.) Merr. Syn.-S. aegyptiaca Pers. (Hindi-Jaint, Jayanti; Englishcommon sesban, Aegyptian Rattle pod) belonging to family-leguminoceae, is a soft wooded, fast growing, short lived shrub,1.8-6 m. high found cultivated throughout the plains of India up to an altitude of 1200 m. and has been reported by Saha[17] et al., 1961; Chaudhury[5], 1966 and Malhi & Trivedi[11], 1972. Bhadu...
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ژورنال
عنوان ژورنال: Brazilian Journal of Chemical Engineering
سال: 2000
ISSN: 0104-6632
DOI: 10.1590/s0104-66322000000400044